A Ti₃C₂/TpPa-1/Cu₂O composite material with high-efficiency bactericidal properties was prepared by in-situ growing TpPa-1-COF on Ti3C2-MXene and anchoring it with Cu₂O NPs. The heterostructure between TpPa-1 and Cu₂O regulated the energy band arrangement and an anti-blocking layer accelerated carrier flow, while Ti3C2 acted as a co-catalyst to promote carrier migration. The Ti3C₂/TpPa-1/Cu2O nanocomposites showed excellent antibacterial rates against S. aureus and P. aeruginosa, increasing by 50% and 33% compared with pure TpPa-1-COF.